Ionic Polymers Based on Dextran: Hydrodynamic Properties in Aqueous Solution and Solvent Mixtures. The value of qM-H varies from one sample to another one, because it is a function of a and the average molecular weights (Mv, Mw). Gekko, K. (1971). pp13-21. Journal of Physical Chemistry, 58(11), 953-957. (2013). The Viscosity of Dilute Solutions of Long-Chain Molecules. 2003;89(2):31120. ; Talsma, H.; Hennink, W.E. Format), Citation-(EndNote Levin G, Gershonowitz A, Sacks H, Stern M, Sherman A, Rudaev S, Zivin I, Phillip M. Transdermal delivery of human growth hormone through RF-microchannels. Journal of Chemical Education, 49(8), 577-579. Chemical penetration enhancers for transdermal drug delivery systems. WebFluorescent and biotinylated dextrans are routinely employed to trace neuronal projections. Webdextran hydrodynamic radius. Conversely, fluorescein sodium was the compound selected to model hydrophilic, low MW drugs. Kim, D.; Robyt, J. F.; So-Young Lee; Jin-Ha Lee; Young-Min Kim. Chem. Unauthorized use of these marks is strictly prohibited. At the same temperature, the hydrodynamic radius and the intrinsic viscosity are higher for different molecular weights, while these properties decrease with an increase of temperature at the same molecular weight (Figure 1). Ubbelohde, Leo. ; Hogan, M.P. Table 3 shows the intrinsic viscosity for the rest of the dextrans obtained by using the single-point method, measured at different temperatures. A novel correlation for protein diffusion coefficients based on molecular weight on radius of gyration. Characterization and Classification of Dextrans from Ninety-six Strains of Bacteria. However, the results did not show significant regeneration of micropore compared to previous in vivo studies which reported significant regeneration [25]. As intrinsic viscosity of dextrans decreases with an increase in solution temperature; chain flexibility enhances with an increase in temperature (see Table 4) showing that the intrinsic viscosity of dextran (with different molecular weights Mw from 8.8 to 200kDa) decreases linearly with an temperature increase in solution, but change to high molecular weight to hyperbranched macromolecule. The human and pig skin samples were placed in the receiver chamber of the Transdermal diffusion cell system with the SC facing up and the donor chamber was fixed in place. The molecular weight of dextran samples can be obtained by a variety of methods including light scattering, HPLC/SEC, sedimentation, osmometry and end-group analysis. A single frequency laser is directed to the sample contained in a cuvette. For a values from 0-0.5 a rigid sphere is predicted in a ideal solvent, from 0.5-0.8 a random coil in good solvent, and from 0.8-2 a rigid or rod like shape is expected (stiff chain). Dynamic light scattering study confirmed the mono-disperse nature of dextran with hydrodynamic radius of 900 nm. Studies carried out at different ionic strength you can see them in the references [64, 65] and works with different solvents in the references [66, 67]. Bookshelf WebIn this work, hydrodynamic properties are investigated in different dextran aqueous solutions with different molecular weights (8.8-2000kDa), in a temperature range from 20C to 50C. Carbohydrate Polymers, 78, 902-907. Viswanath, D.S. The lack of data on the uniformity of intrinsic viscosity measurements in the system water/dextran, highlights the significant influence of the solvent and temperature [74]. The radius of gyration is typically calculated from static scattering measurements, while the hydro-dynamic radius (or equivalent sphere radius) is deter-mined by dynamic light scattering or other diffusion measurements. Catiker, E.; Guner, A. Viscotek GmbH, Europe. (2008). (1987). 2018;1:10915. Im Tiefkhlgert (-5 bis -30C) lagern und vor Licht schtzen. 2019;26(1):25261. Quintanilla-Pineda M, Achou CG, Daz J, Gutirrez-Falcon A, Bravo M, Herrera-Muoz JI, Pea-Navarro N, Alvarado C, Ibaez FC, Marzo F. Foods. WebDextran 670 GPC Standard Normative Mw - 670000 Da GPC standards can be used for calibration and molecular weight determination. The reason for a nonlinear Mark-Houwink relationship is confirmed by the intrinsic viscosity which in contrast with scaling prediction shows no power law behavior. HSE and DK helped to porate the skin and to measure TEWL. Although, intrinsic viscosity is a molecular parameter that can be interpreted in terms of molecular conformation, it does not offer as high resolution on molecular structure as other methods such as Light Scattering, Circular Dichroism, Sedimentation Velocity, Sedimentation Equilibrium, Size Exclusion Chromatography Coupled to Multi-Angle Laser Light Scattering (SEC-MALLS), High-performance Size Exclusion Chromatography (HPSEC), Gel Permeation Chromatography (GPC), NMR, X-rays, etc. Springer Nature. Huang S, Huang G. Preparation and drug delivery of dextran-drug complex. Biophysical Journal, 27, 177-181. FITC-derivatives have been used for a variety of biological applications for more than 40 yeras. This conjecture found to be correct. 4, 10, and 20K) was measured using the Transdermal diffusion cell system. Colloid and Polymer Science, 286, 1223-1231. Determination of branching in biopolymers by size exclusion chromatography with light scattering (Malls), viscosity, and refractive index detection. For simplicity, macromolecules are treated as rigid molecules for a hydrodynamic study. The .gov means its official. Copyright 2006-2023 Thermo Fisher Scientific Inc. All rights reserved, Dextran, Tetramethylrhodamine, 2,000,000 MW, Lysine Fixable, Sulen und Kartuschen fr die Chromatographie, Kunststoffartikel und Zubehr fr das Labor, Spektroskopie, Element- und Isotopenanalyse, Alle Themen fr Hilfe und Support anzeigen, Status und Nachverfolgung von Bestellungen, Reagenzien und Kits fr die Neurowissenschaft, Discover how to track and trace cells by imaging , Hier finden Sie unsere brigen Tracking-Produkte fr Sugetier-Zellkulturen, Molecular Probes Dextran-Konjugate Tabelle14.4. Jeanes, A.; Haynes, W.C.; C. Wilham, A.; Rankin, J.C; Melvin, E.H.; Austin, M. J.; Cluskey, J. E.; Fisher, B.E. Red blood cell rouleaux formation in dextran solution: dependence on polymer conformation. Sachdeva V, Banga AK. Federal government websites often end in .gov or .mil. Polymer molecular weight distribution. Carbohydrate Polymers, 39, 377-380. ; van Nostrum, C.F. Fusco V, Quero GM, Cho GS, Kabisch J, Meske D, Neve H, Bockelmann W, Franz CM. For data requests, please contact the authors. In this work, the human cadaver skin was treated to confirm the skin regeneration after the application of the microporation device. This site needs JavaScript to work properly. In a good solvent, a temperature increase results in an intrinsic viscosity decrease and in a less-extended conformation (RH<), because the entropy value increases with an increase in temperature and it is unfavorable for an extended conformation. Masuelli, Martin Alberto (2013). Deng H, Konopka CJ, Prabhu S, Sarkar S, Medina NG, Fayyaz M, Arogundade OH, Vidana Gamage HE, Shahoei SH, Nall D, Youn Y, Dobrucka IT, Audu CO, Joshi A, Melvin WJ, Gallagher KA, Selvin PR, Nelson ER, Dobrucki LW, Swanson KS, Smith AM. Transdermal drug delivery system for non-steroidal anti inflammatory drugs: a review. Chem. In addition, we evaluated the regeneration of SC after microporation. Dextran Triple Detector Application Note 1, Viscotek GmbH, USA. Chen Rong Huei; Wei Yu Chen; Shang Ta Wang; Chu Hsi Hsu; Min Lang Tsai (2009). 2015;32(5):170413. (1998). Solvent effects on polysaccharide conformation. (2001). (2003). Lee WR, Shen SC, Sun CK, Aljuffali IA, Suen SY, Lin YK, Wang JJ, Fang JY. Dextran Specifications: Label (Ex/Em): Fluorescein (494/521) Size: 40,000 MW. Experimental Review on Intrinsic Viscosity Measurements and Temperature Effect. Although, in literature there is much information on hydrodynamic measurements from determinations viscosity; very few of them evaluate the situation at different temperatures. Microporation was performed 2, 5, and 10 times on each sample. Although we didnt conduct assay for inflammation, we could not find any inflammation symptoms such as redness, blisters, cracked, and thickening. HHS Vulnerability Disclosure, Help and transmitted securely. Journal of Pharmaceutical and Biomedical Analysis, 41, 1260-1267. 2017 Dec 12;27(1):73-78. doi: 10.1007/s10068-017-0274-0. Garcia de la Torre, J.; Carrasco, B. statement and Therefore, it is suitable as model molecules with different molecular weights for transdermal drug delivery [29]. Changes in the Mark-Houwink hydrodynamic volume of chitosan molecules in solutions of different organic acids, at different temperatures and ionic strengths. Drug Deliv Syst. Cite this article. Dextran is soluble in water, methyl sulphoxide, formamide, ethylene glycol, glycerol, 4-methylmorpholine-4-oxide, and hexamethyl phosphamide [4]. Before The molecular weight and Simha number do not change in this temperature range (Mw from 8.8-200kDa), P slightly change, showing modifications in the hydrodynamic properties of the biopolymer in aqueous solution as [] and RH. The effectiveness of the RF microporation technique in transdermal drug delivery of macromolecules and hydrophilic agents such as peptides, hormones, and vaccines has been demonstrated extensively in previous studies [16,17,18]. Chen Rong Huei; Min Lang Tsai (1998). With FITC-dextran 20 K, cumulative The Mark-Houwink a constant is close to 0.75 (or higher) for these good solvents. Journal of Controlled Release, 119, 5-24. Named after George Gabriel Stokes, it is closely related to solute mobility, factoring in not only size but also solvent effects.A smaller ion with stronger hydration, for example, may have a greater Stokes radius than a larger ion with The receptor chamber was filled with PBS. Hydrodynamic radii ( RH) were determined by dynamic light scattering. It is a measure of the width of molecular weight distributions (MWD) as well as probability of molecular weight distribution curve (GPC). Quantitative data are presented as the meanstandard deviation, and comparisons were carried out using one-way ANOVA (Systat Software Inc., Chicago, IL, USA). Environ Toxicol Pharmacol. The Mark-Houwink parameters indicate the dependence with temperature (T) in the range from 20 to 50C, ie with increasing T a increases and kM-H decreases. WebFITC Products. Strategies to improve the potential of transdermal devices by enhancing the skin permeation of therapeutic entities. a Microscopy images of micropore and b quantified data using ImageJ after 0 (immediately), 4, 8, and 24h according to the number of times microproration was done (n=150, *: p<0.05). Determination of the intrinsic viscosity has been the most used measurement in the last 60 years. Webprotein hydrodynamic radius was observed with increasing protein concentration (in the range 0.25-2 mM) in both the absence and presence of 8 M urea. ; Tsuchiya, H.M.; Rist, C.E. Masuelli, Martin Alberto. Advances in Carbohydrate Analysis, 1, 63-144. 7 8 Santoro MM, Gaudino G. Cellular and molecular facets of keratinocyte reepithelization during. Therefore, the trend of "a" and kM-H decreases with T, where the presence of ethylene glycol or dimethylsulfoxide strongly changes the interactions of this solvent with the macromolecule. Differences were considered statistically significant at p <0.05. Format), Citation-(BibTeX The fast increasing of these polyglucosans for medical, industrial and research purposes motivated a survey of the types obtainable. On single point determination of intrinsic viscosity. Morris, G.A. European Polymer Journal, 43, 1744-1753. Corp. (Seoul, Korea). ; Picout, D.R. 2005;22(4):5505. The regeneration process of the skin is regulated by growth factors such as keratinocyte growth factor and fibroblast growth factor, which trigger the proliferation and migration of cells [23, 24]. Dextran molecular size and degree of branching as a function of sucrose concentration, pH, and temperature of reaction of. Crini, G. (2005). The effect of branching is best recognized from the molecular weight dependencies of []. The most widely used method for the characterization of macromolecules is the capillary viscometry, as it is a simple and economic method. It has been found that, when applied to water treatment, it can effectively act on refractory organic matter in wastewater through thermal decomposition and water molecular action to produce free 2008;26(11):12618. government site. Bahary, W. S.; Hogan, M. P.; Jilani, M.; Aronson, M. P. (1995). All possible degrees of curling can be displayed by any molecule, but there will be an average configuration which will depend on the solvent.

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